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    vacuumed for 4 h to form a lipid film. After the sample vial was topped off with sulfur hexafluoride gas, the lipid film was rehydrated with 1 ml of phosphate buffered saline (PBS) containing 10 vol% glycerol and 10 vol% propylene glycol in the heated water bath (50 °C) under the stirring for 1 h.

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    aly), a composition of a core of sulfur hexafluoride (SF ) gas and an envelope of phospholipids, was reconstituted in saline solution according to the manufacturerpro-tocol, and yielded a preparation containing - × mi-crobubbles (MBs)/mL by inversion/agitation of the unit. The average diameter of the MBs was . - . μm.

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    Feb 22, 2016 · The MBs used in this study were commercial sulfur hexafluoride microbubbles (SonoVue, Bracco, Italy), which were approved for routine use in clinical ultrasonography in China. 5 ml sterile saline was added into the vial before use to form a liquid solution by vigorous vibration.

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